Ziehl's Basic Phenified Fuchsin Dye
The stains used in Bacteriology are used to help the morphological visualization of bacteria, which due to their insignificant size are practically transparent.
- Simple coloration with Basic Fenicada Fuchsin facilitates the observation of germs, which stand out for their reddish-pink color. Although it is basically used in simple stains, it is also useful in certain compound stains (Ziehl-Neelsen).
- It is advisable to carry out the staining from young cultures (24-36 h).
- Exclusive use as laboratory reagent Supplied with explanatory leaflet of the instructions for use. Presented in opaque amber PET bottles with a white sealed cap.
SIMPLE COLORING WITH BASIC PHENIC FUCSIN
Method:
- Take a clean and degreased slide. (see codes D100001, D100002, etc.)
- Make a fine and homogeneous spread. It can be made, directly from a broth culture, of a bacterial suspension obtained by homogenizing 1-2 colonies in saline solution or, from a direct sample of a patient.
- Let dry, and fix the extension by passing the slide over the flame of a bunsen burner. Avoid overheating.
- Place the slide on a staining rack (see codes S001, S002, S003)
- Stain the smear by flooding the slide with carbol fuchsin for 2 minutes.
- Wash with water with the help of a washing bottle (eg codes 443000 or 191638), until the washing liquid has no red-pink color.
- Allow to drain vertically (see drainers codes CP24 or CP30)
- Visualize with an immersion objective.
Reference 805120:
- Hazard: h425, H312, H302, H319, H315, H341
- Transportation rules: They do not cost.
Minimum unit of sale: 1 bottle or kit. Consult with the commercial department the special transport conditions.